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A monitoring study on selenium concentration and selenium speciation in plant species of mine areas was conducted in 2015. The results revealed that the average selenium concentration in leaves, stems and roots from five mountain species was significantly higher than that in soils, which ranged from 0.29 to 7.35, 0.05 to 4.46 and 1.08 to 7.05 mg x kg(-1) dry weight, respectively. The selenium concentration in fresh roots (11.9-52.8 mg x kg(-1) dry weight) was significantly higher than that in the roots of four selenium-accumulated species (6.7-24.3 mg x kg(-1) dry weight). Five selenium species, namely, SeO3(2-), H2SeO3(2-), SeO3(2-), Se2O3(4-) and SeO4(2-), were determined by anion exchange chromatography with UV spectrophotometry. The relative abundance of these five selenium species in roots was from 5.2% to 47.6%, and the largest proportion was H2SeO3(2-), which accounted for 47.6% in the three species at the Hejia-Zhushan mining area. The different selenium species contents in leaves, stems and roots revealed that roots and leaves were the main accumulation organs in selenium. The selenium enrichment factor in the different mountain species was much higher than that in the areas without mining, which ranged from 3.1 to 4.0 and 2.9 to 3.4 in stems and leaves, respectively, and from 2.4 to 3.1 and 1.8 to 2.7 in roots and stems, respectively. This study indicated that the selenium enrichment factor of mountain species was about four times that of the non-contaminated areas, and the enrichment factor of selenium in the mountain species
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