Quality Toolbox By Nancy R. Tague.pdf

Quality Toolbox By Nancy R. Tague.pdf

Quality Toolbox By Nancy R. Tague.pdf

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Quality Toolbox By Nancy R. Tague.pdf

The Quality Toolbox 2nd Edition Nancy R Tague.pdf
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Title

:

The Quality Toolbox 2nd Edition

Author

:

Nancy R. Tague

Publisher

:

American Society for Quality – 2006-12

Language

:

English

ISBN-13

:

You must register with us as either a Registered User before you can Download this Book. You’ll be greeted by a simple sign-up page.

Once you have finished the sign-up process, you will be redirected to your download Book page.Development of antisense oligodeoxynucleotides against proliferating cell nuclear antigen (PCNA) as anti-HIV agents.
Antisense oligodeoxynucleotides (oligos) against the polymerase delta interacting protein (PCNA) were prepared using a number of synthetic strategies. Their effectiveness as inhibitors of HIV replication was examined by measuring the inhibition of HIV-1 reverse transcriptase (RT) activity in vitro and the inhibition of cell growth in TZM-bl cells. Each of the oligos was also evaluated for their ability to modulate the HIV-1 long terminal repeat (LTR) expression in cells or the RNA of intracellular HIV-1 structural genes in vivo. Most of the oligos under investigation inhibited both HIV-1 RT activity and cell growth, and several were found to affect HIV-1 gene expression in cultured cells or in mice. However, most of them did not have a significant effect on the intracellular HIV-1 RNA levels in TZM-bl cells. Although a slight inhibition of viral gene expression by some of the oligos in vivo was observed, when the oligos were injected into mice, they did not seem to affect the ability of HIV-1 to establish a latent infection in vivo. Together, these results demonstrated the effectiveness of antisense oligos against PCNA as potent inhibitors of HIV replication, but also indicated that PCNA is an important host target for the development of new anti-HIV agents.Q:

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